However, some of the disadvantages for tissue clearance are that immunostaining of archival tissues is usually complicated as a result of the antigen masking due to formaldehyde protein cross-linking 7. Clearing techniques can be very helpful in attempting to explain neuron networks in greater brain volumes, such as the adult mouse brain, in an entire state without disassembly 5, 6. In life science, EM is widely used to explore the subcellular components, which are several orders of magnitude smaller than the spatial neuron networks. Several techniques have been developed to study biological structure in 3D like serial block-face scanning electron microscopy (EM), focused ion beam scanning EM, serial-section transmission EM, automatic tape-collecting ultramicrotome-scanning EM, and many types of light microscopy with or without tissue clearing 1, 2, 3, 4. Life science aims at a better understanding of multiple biological functions, such as healthy organ development with cellular proliferation, migration and organization, tumor formation, and general pathology.
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